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endoglycosidase h  (New England Biolabs)


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    Structured Review

    New England Biolabs endoglycosidase h
    Endoglycosidase H, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4345 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endo+h/Endo+H/pmc12753284-168-41-43
    Average 99 stars, based on 4345 article reviews
    endoglycosidase h - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Residue:

    Article Title: Induction of broadly neutralizing HIV antibodies by a two-step mechanism informs vaccine design.
    Article Snippet: .. The remaining glycopeptides were first digested with Endo H (New England Biolabs) to deplete oligomannose- and hybrid-type glycans and leave a single GlcNAc or GlcNAcFuc residue at the corresponding site. .. The reaction mixture was then dried completely and resuspended in a mixture containing 50 mM ammonium bicarbonate and PNGase F (New England Biolabs) using only H2O18 (Sigma-Aldrich) throughout.

    Incubation:

    Article Title: Discovery of Autophagy Modulators that Increase I1061T NPC1 Expression and Promote Cholesterol Efflux in Niemann-Pick Type C Patient-Derived Fibroblasts
    Article Snippet: For the PNGase F reaction, 2 μL 10x GlycoBuffer 2 (NEB, B3704SVIAL), 2 μL 10% NP-40 (NEB, B2704SVIAL), and 6 μL H2O were added to the reaction vial to make a final volume of 20 μL. .. The samples were briefly spun down, then 0.5 μL of PNGaseF stock (NEB, P0704SVIAL) was added to the reaction and incubated at 37 °C for 3 h. For the Endo H and NT samples, 2 μL of 10x GlycoBuffer 3 (NEB, B1720SVIAL), 0.75 μL Endo H (NEB, P0702SVIAL), and ultrapure H 2 O were added to the reaction vial to bring total volume to 20 μL. ..

    Article Title: A delayed translocation into the endoplasmic reticulum controls the post-translational modifications of PD-L1.
    Article Snippet: .. For the deglycosylation digestion, 10 μg of protein extracts were treated according to the manufacturer's instructions and incubated for 1 h at 37°C with 500 U of Endo H, 500 U of PNGase F or 50 U O-glycosidase (New England BioLabs). ..

    Glycoproteomics:

    Article Title: Antibodies specific to glycosylated LAG3 and methods of use thereof
    Article Snippet: .. To confirm glycosylation of LAG3 protein, cell lysates were treated with the enzymes PNGase F, Endo H, 0-glycosidase (New England BioLabs, Ipswich, MA, USA) as described by the manufacturer. ..

    Article Title: Mannosylated nanoparticle immunogens enhance the circumsporozoite protein-specific B cell response and improve protection against sporozoite challenge
    Article Snippet: .. After denaturation, the N-linked glycan content of glycosylated RT.2-I53-50A components or nanoparticles was estimated by treatment with PNGase F (NEB) which cleaves all N-linked glycoforms, and Endo H (EndoH, NEB) which only cleaves oligomannose. ..



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    A) After treatment of MDCKI-hNKCC2 cells with tetracycline, RT-PCR with primers against hNKCC2A and 18S rRNA (control) demonstrated robust NKCC2 mRNA expression. Immunoblotting of MDCKI-hNKCC2 cells using a flag-tag antibody demonstrated relatively low NKCC2 protein levels. B) Representative immunoblots using flag-tag or 20S proteasome (control) antibodies of MDCKI-hNKCC2A cells pretreated with 5 or 15 mM valproic acid (VpA) for 24 h prior to tetracycline (Tet) induction. C) Immunoblots of MDCKI-hNKCC2A cells treated with tetracycline combined with VpA for various time points. D) Representative immunoblots of lysate from tetracycline and VpA induced MDCKI-hNKCC2A cells grown on semi-permeable supports subjected to apical surface biotinylation. Total and biotinylated pools were blotted with flag-tag or 20S proteasome antibodies. E) Representative confocal laser micrograph (top, xy plane; bottom, xz plane) of tetracycline and VpA induced MDCKI-hNKCC2A cells grown on semi-permeable supports labeled with flag-tag antibody. F) NKCC2 was immunoprecipitated (IP) from MDCKI-hNKCC2 cells using a flag-tag antibody, with negative controls performed with or without addition of antibody or lysate. Immunoprecipiates were blotted for flag (NKCC2) or total ubiquitin (Ubi). G) NKCC2 exists as a highly complex glycosylated protein. Representative immunoblots using flag-tag and 20S proteasome (control) antibodies of lysates from tetracycline and VpA induced MDCKI-hNKCC2 cells treated with PNGase F (1 or 2 µl) or EndoH (2 or 5 µl) for 1 h at 37°C.

    Journal: bioRxiv

    Article Title: Regulation of the Na-K-2Cl cotransporter NKCC2 by ubiquitylation

    doi: 10.64898/2026.05.07.723577

    Figure Lengend Snippet: A) After treatment of MDCKI-hNKCC2 cells with tetracycline, RT-PCR with primers against hNKCC2A and 18S rRNA (control) demonstrated robust NKCC2 mRNA expression. Immunoblotting of MDCKI-hNKCC2 cells using a flag-tag antibody demonstrated relatively low NKCC2 protein levels. B) Representative immunoblots using flag-tag or 20S proteasome (control) antibodies of MDCKI-hNKCC2A cells pretreated with 5 or 15 mM valproic acid (VpA) for 24 h prior to tetracycline (Tet) induction. C) Immunoblots of MDCKI-hNKCC2A cells treated with tetracycline combined with VpA for various time points. D) Representative immunoblots of lysate from tetracycline and VpA induced MDCKI-hNKCC2A cells grown on semi-permeable supports subjected to apical surface biotinylation. Total and biotinylated pools were blotted with flag-tag or 20S proteasome antibodies. E) Representative confocal laser micrograph (top, xy plane; bottom, xz plane) of tetracycline and VpA induced MDCKI-hNKCC2A cells grown on semi-permeable supports labeled with flag-tag antibody. F) NKCC2 was immunoprecipitated (IP) from MDCKI-hNKCC2 cells using a flag-tag antibody, with negative controls performed with or without addition of antibody or lysate. Immunoprecipiates were blotted for flag (NKCC2) or total ubiquitin (Ubi). G) NKCC2 exists as a highly complex glycosylated protein. Representative immunoblots using flag-tag and 20S proteasome (control) antibodies of lysates from tetracycline and VpA induced MDCKI-hNKCC2 cells treated with PNGase F (1 or 2 µl) or EndoH (2 or 5 µl) for 1 h at 37°C.

    Article Snippet: Protein concentrations were measured using BCA assays (Thermo) and equal protein amounts were denatured in 1× denaturing buffer (New England Biolabs) for 15 min at 65 °C, chilled on ice and centrifuged for 10 sec, before deglycosylation using PNGase F (Peptide N-glycosidase F) or EndoH (Endoglycosidase H) (New England Biolabs) for 1 h using standard protocols.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Expressing, Western Blot, FLAG-tag, Labeling, Immunoprecipitation, Ubiquitin Proteomics